Part of their appeal is that they can grow on sunlight and carbondioxide alone and thus could contribute to lowering greenhouse gas emissions and moving away from a petrochemical-based economy.
However, familiar cyanobacterial strains grow more slowly than
the bacterial and yeast bio-factories already in use, and their genetic and metabolic networks are not as well understood.
So it was exciting news when a group of scientists led by
Himadri B. Pakrasi, PhD, the George William and Irene Koechig
Freiberg professor of biology in Arts & Sciences at Washington
University in St. Louis, reported in the Sept. 30 issue of Scientific Reports that they have identified a fast growing
cyanobacterial strain, called Synechococcus elongatus UTEX
2973.
Rapid growth may allow this cyanobacterial strain to outcompete contaminating ones and eventually to synthesize larger quantities of biofuel or other valuable products.
It also has the more immediate benefit of making it easier to do the experimental work needed to understand the bacterium well
enough that it can serve as a "chassis" that can be retooled for a variety of purposes.
Because other cyanobacterial strains grow sluggishly, it takes weeks or months to perform experiments with them that can be performed in E. coli or yeast in days.
The newly identified strain might ultimately prove useful for carbon sequestration, biofuel production, biosynthesis of valuable chemicals and the search for novel pharmaceuticals.
"What intrigues me most about these microbes is their
ingenuity," Pakrasi said. "They have somehow figured out how
to multiply rapidly by using sunlight and carbon dioxide very
efficiently." Pakrasi, who also serves as director of Washington
University's International Center for Advanced Renewable
Energy and Sustainability (I-CARES), and has been a prominent
advocate of cyanobacterial synthetic biology for a decade.
Hiding in plain sight
Like the famous purloined letter, the cyanobacterial strain was hiding in plain sight—or to be precise, in a collection of algae cultures at the University of Texas in Austin. (Cyanobacteria are
sometimes called blue-green algae, but this a misnomer.)
Although most cyanobacteria grow slowly, in 1955 two scientists at the University of Texas at Austin described a fast-growing cyanobacterial strain collected from a campus creek. Whereas most strains grew by 5 to 8 percent per hour, this strain
grew by 30 percent per hour. What's more, it grew fastest at the relatively high temperature of 38 degrees C (104 degrees F).
This strain was eventually deposited in the UTEX algae culture collection as Synechococcus leopoliensis UTEX 625. However, at some point the UTEX 625 strain was contaminated
and lost its rapid growth property. The Pakrasi lab obtained a frozen sample of the UTEX strain, and by careful coaxing under appropriate conditions, recovered a pure, fast-growing strain from the mixed culture of the deposited algae.
Under favorable conditions, the newly isolated strain grows at
more than 50 percent per hour, the highest growth rate reported
to date for any cyanobacterial strain, and almost twice as fast as a widely studied close relative. Since the new strain may not be the one that was described in 1955, the scientists deposited it in the
UTEX algae collection as Synechococcus elongatus UTEX
2973.
Kicking the tires on the new model
To characterize the new strain, Washington University sequenced
its genome. To the scientists' surprise, the new strain turned out to be remarkably similar to a widely studied cyanobacterium,
Synechococcus elongatus PCC 7942, originally discovered far
away from Texas (in lakes in California), that grows only half as fast.
Since the genome sequences of the two strains are 99.8 percent
identical, the genetic determinants of rapid growth almost certainly lie in the remaining 0.2 percent.
The proteomes (the set of proteins produced by an organism) of both of these strains were analyzed at the Environmental Molecular Sciences Laboratory, a Department of Energy national scientific user facility located at Pacific Northwest National Laboratory in Richland, Wash. This data, which covers 68 percent of the proteins the microbes produce, will guide further work with both strains.
The scientists also showed that the genome of the new strain can
be easily manipulated, a characteristic essential to its use as a host for projects in synthetic biology.
"Cyanobacteria have the potential to be the ideal biofactories for sustainable carbon negative production of numerous compounds," Pakrasi said. "This fast-growing strain should help to realize that dream."
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